LIST OF CONTRIBUTORS xi (6)
PREFACE xvii
Section I. Purification, Functional 3 (278)
Characterization, Reconstitution, and
Organization
A. Animals 3 (170)
1. Long-Term Preservation of Liver for 3 (4)
Subcellular Fractionation
SIDNEY FLEISCHER
MARIJA KERVINA
2. Subcellular Fractionation of Rat Liver 7 (36)
SIDNEY FLEISCHER
MARIJA KERVINA
3. The Use of Perfusion of Liver and Other 43 (12)
Organs for the Study of Microsomal
Electron-Transport and Cytochrome P-450
Systems
HELMUT SIES
4. Isolation and Use of Liver Cells 55 (12)
PETER MOLDEUS
JOHAN HOGBERG
STEN ORRENIUS
5. Isolation of Rough and Smooth 67 (12)
Microsomes--General
GUSTAV DALLNER
6. Isolation and Characterization of Golgi 79 (12)
Apparatus and Membranes from Rat Liver
BECCA FLEISCHER
7. Isolation of Rat Liver Peroxisomes 91 (14)
PIERRE BAUDHUIN
8. Isolation of Modified Liver Lysosomes 105 (8)
ANDRE TROUET
9. Preparation of Impermeable Ghosts and 113 (8)
Inside-out Vesicles from Human Erythrocyte
Membranes
THEODORE L. STECK
JEFFREY A. KANT
10. Mitochondria from Brown Adipose Tissue: 121 (14)
Isolation and Properties
BARBARA CANNON
OLOV LINDBERG
11. Survey and Update of Outer and Inner 135 (10)
Mitochon-drial Membrane Separation
JOHN W. GREENAWALT
12. Tightly Coupled Beef Heart 145 (8)
Submitochondrial Particles
CHUAN-PU LEE
13. Respiratory Control and Oxidative 153 (6)
Phosphorylation Measurements in Mitochondria
K. VAN DAM
A. H. C. A. WIECHMANN
14. Preparation of Retinal Rod Outer Segments 159 (6)
DAVID S. PAPERMASTER
15. Preparation of a Nuclear Matrix-Pore 165 (8)
Complex--Lamina Fraction from Embryos of
Drosophila melanogaster
PAUL A. FISHER
GUNTER BLOBEL
B. Plants 173 (58)
16. Isolation of Intact Chloroplasts: 173 (14)
General Principles and Criteria of Integrity
DAVID A. WALKER
ZORAN G. CEROVIC
SIMON P. ROBINSON
17. Isolation of Plant Mitochondria: General 187 (14)
Principles and Criteria of Integrity
ROLAND DOUCE
JACQUES BOURGUIGNON
RENAUD BROUQUISSE
MICHEL NEUBURGER
18. Isolation of Glyoxysomes and 201 (12)
Purification of Glyoxysomal Membranes
EUGENE L. VIGIL
TUNG K. FANG
ROBERT P. DONALDSON
19. Isolation of the Plasma Membrane: 213 (18)
Membrane Markers and General Principles
DONALD P. BRISKIN
ROBERT T. LEONARD
THOMAS K. HODGES
C. Bacterial and Unicellular Eukaryotes 231 (50)
20. Preparation of Yeast Mitochondria 231 (12)
(Saccharomcyces cerevisiae) with Good P/O and
Respiratory Control Ratios
BERNARD GUERIN
PIERRE LABBE
MARIE SOMLO
21. Isolation of the Cell Membrane of 243 (12)
Halobacterium halobium and Its Fractionation
into Red and Purple Membrane
DIETER OESTERHELT
WALTHER STOECKENIUS
22. Isolation of Cell Walls from 255 (16)
Gram-Positive Bacteria
MILTON R. J. SALTON
23. The Isolation of Gas Vesicles from 271 (10)
Blue-Green Algae
A. E. WALSBY
Section II. Specialized Enzymes, Assays, and 281 (116)
Reconstitution Methodology
24. Reconstitution of Functional 281 (8)
Sarcoplasmic Reticulum Membrane Vesicles
GERHARD MEISSNER
SIDNEY FLEISCHER
25. Preparation and Assay of Superoxide 289 (12)
Dismutases
JAMES D. CRAPO
JOE M. MCCORD
IRWIN FRIDOVICH
26. EPR Spectroscopy of Components of the 301 (18)
Mitochondrial Electron-Transfer System
HELMUT BEINERT
27. The Measurement of Difference Spectra: 319 (10)
Application to the Cytochromes of Microsomes
R. W. ESTABROOK
J. WERRINGLOER
28. The Microsomal Ethanol Oxidizing System 329 (14)
(MEOS)
CHARLES S. LIEBER
LEONORE M. DECARLI
SHOHEI MATSUZAKI
KUNIHIKO OHNISHI
ROLF TESCHKE
29. Steroid Hydroxylations Catalyzed by 343 (12)
Cytochrome P-450
JAN-AKE GUSTAFSSON
30. Delipidation, Renaturation, and 355 (8)
Reconstitution of Bacteriorhodopsin
HAGAN BAYLEY
BO HOJEBERG
KUO-SEN HUANG
H. GOBIND KHORANA
MEI-JUNE LIAO
CHRISTINA LIND
ERWIN LONDON
31. Morphometry of Subcellular Fractions 363 (18)
P. BAUDHUIN
32. Application of the Freeze-Fracture 381 (10)
Technique to Natural membranes
K. FISHER
D. BRANTON
33. Optical Properties of Cytochromes from 391 (6)
Beef Heart Mitochondria, Submitochondrial
Vesicles, and Derived Preparations
B. F. VAN GELDER
Section III. Membrane Lipids and Lipoproteins 397 (62)
34. Sensitive Protein Assay in Presence of 397 (8)
High Levels of Lipid
RONALD S. KAPLAN
PETER L. PEDERSEN
35. Phosphatidylcholine Exchange Protein 405 (8)
from Beef Liver
H. H. KAMP
K. W. A. WIRTZ
36. Microsomal Lipid Peroxidation 413 (10)
JOHN A. BUEGE
STEVEN D. AUST
37. Glutathione Peroxidase and Hydroperoxides 423 (8)
A. L. TAPPEL
38. Liposome Preparation and Size 431 (28)
Characterization
MARTIN C. WOODLE
DEMETRIOS
PAPAHADJOPOULOS
Section IV. Biogenesis, Targeting, and Assembly 459 (138)
39. Methods for the Preparation of Messenger 459 (14)
RNA
PAUL M. LIZARDI
40. Preparation of Microsomal Membranes for 473 (10)
Cotranslational Protein Translocation
PETER WALTER
GUNTER BLOBEL
41. Quantitative Assay for Signal Peptidase 483 (12)
ROBERT C. JACKSON
42. Isolation and Properties of the Porin of 495 (8)
the Outer Mitochondrial Membrane from
Neurospora crassa
HELMUT FREITAG
ROLAND BENZ
WALTER NEUPERT
43. Import of Polypeptides into Isolated 503 (8)
Yeast Mitochondria
SUSAN M. GASSER
44. Analysis of Yeast Mitochondrial Genes 511 (14)
CAROL L. DIECKMANN
ALEXANDER TZAGOLOFF
45. Analysis of Human Mitochondrial RNA 525 (36)
GIUSEPPE ATTARDI
JULIO MONTOYA
46. In Vitro Reconstitution of Synthesis, 561 (8)
Uptake, and Assembly of Cytoplasmically
Synthesized Chloroplast Proteins
JHON E. MULLET
NAM-HAI CHUA
47. Receptor-Mediated Endocytosis of 569 (20)
Low-Density Lipoprotein in Cultured Cells
JOSEPH L. GOLDTEIN
SANDIP K. BASU
MICHAEL S. BROWN
48. Binding, Endocytosis, and Degradation of 589 (8)
Enveloped Animal Viruses
MARK MARSH
ARI HELENIUS
KARL MATLIN
KAI SIMONS
Section V. Bioenergetics and Transport 597 (474)
49. Isolation of Transport Mutants in 597 (8)
Bacteria
GIOVANNA FERRO-LUZZI AMES
50. Reconstitution of the Electron-Transport 605 (8)
System of Bovine Heart Mitochondria
YOUSSEF HATEFI
51. Uncouplers of Oxidative Phosphorylation 613 (12)
PETER G. HEYTLER
52. The Measurement of Membrane Potential 625 (24)
and pH in Cells, Organelles, and Vesicles
HAGAI ROTTENBERG
53. Measurement of -->H+/O in Mitochondria 649 (14)
and Submitochondria Vesicles
PETER MITCHELL
JENNIFER MOYLE
ROY MITCHELL
54. Determination of the H+/Site and 663 (16)
Ca2+/Site Ratios of Mitochondrial Electron
Transport
BALTAZAR REYNAFARJE
MARTIN D. BRAND
ADOLFO ALEXANDRE
ALBERT L. LEHNINGER
55. Direct Methods for Measuring Metabolite 679 (24)
Transport and Distribution in Mitochondria
FERDINANDO PALMIERI
MARTIN KLINGENBERG
56. Continuous Measurement of Adenosine 703 (14)
Triphosphate with Firefly Luciferase
Luminescence
JOHN J. LEMASTERS
CHARLES R. HACKENBROCK
57. Fluorescent Nucleotide Analogs as Active 717 (14)
Site Probes for the ADP/ATP Carrier and the
Uncoupling Protein
MARTIN KLINGENBERG
58. Transport of Metabolites across the 731 (12)
Chloroplast Envelope
H. W. HELDT
U. I. FLUGGE
59. Isolation and Reconstitution of CF0-F1 743 (8)
from Chloroplasts
URI PICK
60. Resonance Raman Methods for Proton 751 (12)
Translocation in Bacteriorhodopsin
MARK S. BRAIMAN
61. Measurement of Transmembrane Proton 763 (8)
Movements with Nitroxide Spin Probes
ROLF J. MEHLHORN
LESTER PACKER
ROBERT MACEY
ALEXANDRU T. BALABAN
ILEANA DRAGUTAN
62. Reconstitution of an H+ Translocator, 771 (8)
the "Uncoupling Protein" from Brown Adipose
Tissue Mitochondria, in Phospholipid Vesicles
MARTIN KLINGENBERG
EDITH WINKLER
63. Purification of Na+, K+ -ATPase: Enzyme 779 (16)
Sources, Preparative Problems, and
Preparation from Mammalian Kidney
PETER LETH JORGENSEN
64. Measurement of Active and Passive Na+ 795 (10)
and K+ Fluxes in Reconstituted Vesicles
S. J. D. KARLISH
65. Isolation of Sarcoplasmic Reticulum 805 (10)
Fractions Referable to Longitudinal Tubules
and Junctional Terminal Cisternae from Rabbit
Skeletal Muscle
ALICE CHU
MARK C. DIXON
AKITSUGU SAITO
STEVEN SEILER
SIDNEY FLEISCHER
66. Isolation of Canine Crediac Sarcoplasmic 815 (10)
Reticulum
BRIAN K. CHAMBERLAIN
SIDNEY FLEISCHER
67. Reconstitution of Calcium Pumping of 825 (8)
Cardiac Sarcoplasmic Reticulum
MAKOTO INUI
SIDNEY FLEISCHER
68. Purification, Reconstitution, and 833 (12)
Regulation of Plasma Membrane Ca2+-Pumps
JOHN T. PENNISTON
ADELAIDA G. FILOTEO
CAROL S. MCDONOUGH
ERNESTO CARAFOLI
69. Purification of Ca2+ Release Channel 845 (16)
(Ryanodine Receptor) from Heart and Skeletal
Muscle Sarcoplasmic Reticulum
MAKOTO INUI
SIDNEY FLEISCHER
70. Purification of Vacuolar Membranes, 861 (12)
Mitochondria, and Plasma Membranes from
Neurosopora crassa and Modes of
Discriminating among the Different H+-ATPases
EMMA JEAN BOWMAN
BARRY J. BOWMAN
71. Chromaffin Granule Proton Pump 873 (16)
NATHAN NELSON
SHULAMIT CIDON
YOSHINORI MORIYAMA
72. Proton Pump of Clathrin-Coated Vesicles 889 (12)
XIAO-SONG XIE
DENNIS K. STONE
EFRAIM RACKER
73. Preparation of Gastric H+, K+-ATPase 901 (6)
EDD C. RABON
WHA BIN IM
GEORGE SACHS
74. Measurement of Cytosolic Free Ca2+ with 907 (34)
Quin2
ROGER TSIEN
TULLIO POZZAN
75. Cation Fluxes in the Red Blood Cell: 941 (14)
Na+, K+ Pump
JOHN R. SACHS
76. Sugar Transport in Red Blood Cells 955 (20)
W. F. WIDDAS
77. Transport of Glutathione, Glutathione 975 (12)
Disulfide, and Glutathione Conjugates across
the Hepatocyte Plasma Membrane
THEO P. M. AKERBOOM
HELMUT SIES
78. Glutamate Accumulation into Synaptic 987 (18)
Vesicles
PHILLIP E. KISH
TETSUFUMI UEDA
79. Calcium Ion Transport in Mitochondria 1005(18)
NILS-ERIK LEO SARIS
ASHLEY ALLSHIRE
80. Purification and Reconstitution of 1023(12)
Epithelial Chloride Channels
DONALD W. LANDRY
MYLES A. AKABAS
CHRISTOPHER REDHEAD
QAIS AL-AWQATI
81. Isolation of H+, K+ -ATPase-Containing 1035(16)
Membranes from the Gastric Oxyntic Cell
W. W. REENSTRA
J. G. FORTE
82. Patch-Clamp Experiments in Epithelia: 1051(16)
Activation by Hormones or Neurotransmitters
OLE H. PETERSEN
83. Epithelial Transport: An Introduction 1067(4)
KARL JULIUS ULLRICH
INDEX 1071
|
ISBN:9780125435659
(Paper cover book) 1095 p.